IHC is the localization of antigens in tissue sections by the use of labeled antibodies as specific reagents through antigen-antibody interactions that are visualized by a marker such as Fluorescent dye,Enzymes
The basic principle of any IHC procedures is that an antibody will specifically bind with an antigen to produce an exclusive antibody-antigen complex.
This bonding is used to visualize both normal and diseased states of tissues, infectious agents and other components that may not be demonstrated by histochemical or special stains.
Special positive controls are tested with each test in order to test the staining protocol and for specificity of the antibody being used.
Negative control are involved in the run .
The section Member in IHC National External Quality Scheme.UK