Enzyme histochemical studies are the gold standard of diagnosis in muscle diseases.
Fresh Muscle Biopsy is required to perform the tests
The Aims of the studies:
To find out the distribution and changes in muscle fibers.
To rule out Mitochondrial related diseases.
Types of Staining:
1- ATPase present in muscle tissue hydrolyzes the substrate forming ADP and phoshate group. Exposing the tissue to cobalt chloride and present on the enzyme activity site and colored by ammonium sulfide with black.
Fig1. ATPase enzyme activity study
2- NADH and SDH
Dehydrogenase enzyme act on the substrate to release hydrogen the reduces tetrazolium salt on activity site showing blue color
Fig2. SDH enzyme activity study