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Immunofluorescence (IF)


Is a technique based on antigen – antibody reaction.  A fluorescent dye known as fluorochrome is conjugated to it, most commonly will be a green apple when examined under fluorescent microscope. It is used to examine fresh renal and skin biopsies. It could be direct, where only one antibody is directed against the antigen or indirect where a primary antibody is directed against the antigen then a labeled secondary antibody is directed against the primary antibody.



Fig1. Renal glomeruli detected using IF